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allintm hs red taq mastermix 2x  (highQu Inc)


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    Structured Review

    highQu Inc allintm hs red taq mastermix 2x
    Allintm Hs Red Taq Mastermix 2x, supplied by highQu Inc, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/allintm+hs+red+taq+mastermix/ALLin+HS+Red+Taq+Mastermix/pmc11509576-117-16-22
    Average 94 stars, based on 21 article reviews
    allintm hs red taq mastermix 2x - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Different impact of bovine complement regulatory protein 46 (CD46 bov ) as a cellular receptor for members of the species Pestivirus H and Pestivirus G
    Article Snippet: In order to characterize the CRISPR/Cas9-induced genome alterations of CD46 bov in the MDBKΔCD46 cell line, cellular RNA was extracted from MDBK wild type (WT) and MDBKΔCD46 cells using the KingFisherTM Duo Prime Purification System (Thermo Fisher Scientific) and the IndiMag® Pathogen Kit (SP947457, Indical Bioscience) according to the manufacturers’ instructions. cDNA synthesis was performed with SuperScriptTM III Reverse Transcriptase (18080093, Invitrogen) and random hexamer primers (48190011, Invitrogen). .. To obtain templates for sequencing, a 731 bp amplicon was generated using the ALLinTM HS Red Taq Mastermix (HSM0305, highQu) and a primer pair flanking the guide RNA target site (bovCD46_14-fw and bovCD46_744-rev, ). .. The following thermal profile was applied: 95°C for 3 min, 40 cycles of 95°C for 15 sec, 56°C for 30 sec and 72°C for 30 sec, and final extension at 72°C for 5 min. PCR amplicons were purified using the GeneJET PCR Purification Kit (K0702, Thermo Fisher Scientific).

    Amplification:

    Article Title: Different impact of bovine complement regulatory protein 46 (CD46 bov ) as a cellular receptor for members of the species Pestivirus H and Pestivirus G
    Article Snippet: In order to characterize the CRISPR/Cas9-induced genome alterations of CD46 bov in the MDBKΔCD46 cell line, cellular RNA was extracted from MDBK wild type (WT) and MDBKΔCD46 cells using the KingFisherTM Duo Prime Purification System (Thermo Fisher Scientific) and the IndiMag® Pathogen Kit (SP947457, Indical Bioscience) according to the manufacturers’ instructions. cDNA synthesis was performed with SuperScriptTM III Reverse Transcriptase (18080093, Invitrogen) and random hexamer primers (48190011, Invitrogen). .. To obtain templates for sequencing, a 731 bp amplicon was generated using the ALLinTM HS Red Taq Mastermix (HSM0305, highQu) and a primer pair flanking the guide RNA target site (bovCD46_14-fw and bovCD46_744-rev, ). .. The following thermal profile was applied: 95°C for 3 min, 40 cycles of 95°C for 15 sec, 56°C for 30 sec and 72°C for 30 sec, and final extension at 72°C for 5 min. PCR amplicons were purified using the GeneJET PCR Purification Kit (K0702, Thermo Fisher Scientific).

    Article Title: One-Step Soft Agar Enrichment and Isolation of Human Lung Bacteria Inhibiting the Germination of Aspergillus fumigatus conidia
    Article Snippet: .. The 16S rRNA gene was amplified using the ALLinTM HS Red Taq Mastermix, 2X kit (highQu, Germany) with the GM3f (5 ́-AGAGTTTGATCMTGGC-3 ́)-GM4r (5 ́-TACCTTGTTACGACTT-3 ́) primer pair [22]. ..

    Article Title: One-Step Soft Agar Enrichment and Isolation of Human Lung Bacteria Inhibiting the Germination of Aspergillus fumigatus Conidia
    Article Snippet: .. The 16S rRNA gene was amplified using the ALLinTM HS Red Taq Mastermix, 2X kit (highQu, Kraichtal, Germany) with the GM3f (5′-AGAGTTTGATCMTGGC-3′)-GM4r (5′-TACCTTGTTACGACTT-3′) primer pair [ ]. ..

    Generated:

    Article Title: Different impact of bovine complement regulatory protein 46 (CD46 bov ) as a cellular receptor for members of the species Pestivirus H and Pestivirus G
    Article Snippet: In order to characterize the CRISPR/Cas9-induced genome alterations of CD46 bov in the MDBKΔCD46 cell line, cellular RNA was extracted from MDBK wild type (WT) and MDBKΔCD46 cells using the KingFisherTM Duo Prime Purification System (Thermo Fisher Scientific) and the IndiMag® Pathogen Kit (SP947457, Indical Bioscience) according to the manufacturers’ instructions. cDNA synthesis was performed with SuperScriptTM III Reverse Transcriptase (18080093, Invitrogen) and random hexamer primers (48190011, Invitrogen). .. To obtain templates for sequencing, a 731 bp amplicon was generated using the ALLinTM HS Red Taq Mastermix (HSM0305, highQu) and a primer pair flanking the guide RNA target site (bovCD46_14-fw and bovCD46_744-rev, ). .. The following thermal profile was applied: 95°C for 3 min, 40 cycles of 95°C for 15 sec, 56°C for 30 sec and 72°C for 30 sec, and final extension at 72°C for 5 min. PCR amplicons were purified using the GeneJET PCR Purification Kit (K0702, Thermo Fisher Scientific).



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